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Fig. 1 | BMC Research Notes

Fig. 1

From: Contaminating reactivity of a monoclonal CCAAT/Enhancer Binding Protein β antibody in differentiating myoblasts

Fig. 1

Anti-C/EBPβ antibody E299 detects a ~ 23 kDa band in differentiating myoblasts. a C/EBPβ protein expression in proliferating C2C12 myoblasts that were retrovirally transduced to express the LAP isoform of C/EBPβ (β-LAP), the LIP isoform (β-LIP), the full length C/EBPβ (β) or with empty virus (pLXSN). b C/EBPβ protein expression in proliferating (growth medium, GM) or differentiating (differentiation medium, DM, 12–96 h) C2C12 myoblasts. Migration of the unexpected band is indicated by “?”. c C/EBPβ protein expression in proliferating (GM) or differentiating (24–72 h) C2C12 myoblasts that were retrovirally transduced to express C/EBPβ (β) or with empty virus (pLXSN). d C/EBPβ protein expression in proliferating (GM) or differentiating (0–48 h) primary myoblasts isolated from Cebpbfl/flPax7+/+ (WT) and Cebpbfl/flPax7 CreER/+ (cKO) mice

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