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Table 1 Primers and PCR timetable program for sfa, afa, and foc genes in UPEC strain

From: Identification phenotypic and genotypic characterization of biofilm formation in Escherichia coli isolated from urinary tract infections and their antibiotics resistance

Genes

Initial cycle

Denaturation

Annealing

Extension

Final extension

Primers sequencing

Product size (bp)

sfa gene

 Time

4 M

30 S

30 M

40 S

3 M

F:5′ C C G T A A G A T G T C T G C G A G 3′

R:5′ A G C A A G T C T G G C A A C G A G 3′

100

 Temperature

95

95

53

72

72

 Number of cycles

1

35

35

35

1

afa gene

 Time

5 M

1 S

30 M

3 S

7 M

F:5′ G C T G G G C A G C A A A C T G A T A A C T C T C 3′

R:5′ C A T C A A G C T G T T T G T T C G T C C G C C G 3′

750

 Temperature

95

95

60

72

72

 Number of cycles

1

35

35

35

1

foc gene

 Time

4 M

1 S

1 M

2 S

10 M

F:5′ G G T G G A A C C G C A G A A A A T A C 3′

R:5′ G A A C T G T T G G G G A A A G A G T G 3′

388

 Temperature

95

95

58

72

72

 Number of cycles

1

35

35

35

1