Table 1 Spa typing results of the 50 MRSA clinical isolates, using conventional PCR-sequencing method and HRM
Spa types | No. of isolates (n:50) | PCR base spa types | Tm (°C) | GC % | Size bp |
|---|---|---|---|---|---|
t 030 | 7 | 15-12-16-02-24-24 | 83.83 | 44 | 250 |
t 037 | 2 | 15-12-16-02-25-17-24 | 82.16 | 44.6 | 360 |
t 021 | 5 | 15-12-16-02-16-02-25-17-24 | 85.3 | 43.9 | 300 |
t 386 | 4 | 07-23-13 | 82.96 | 42.2 | 210 |
t 325 | 2 | 07-12-21-17-34-13-34-34-33-34 | 82.95 | 41.1 | 400 |
t 345 | 1 | 26-23-13-21-17-34-34-33-34 | 85.18 | 41.7 | 350 |
t 790 | 1 | 26-23-13-23-31-29-17-25-17-25-16-28 | 84.13 | 44.1 | 450 |
t 314 | 1 | 08-17-23-18-17 | 85.66 | 44.6 | 270 |
t 186 | 1 | 07-12-21-17-13-13-34-34-33-34 | 84.5 | 41.8 | 300 |
t 304 | 1 | 11-10-21-17-34-24-34-22-25 | 84.35 | 42.8 | 300 |
t 003 | 1 | 26-17-20-17-12-17-17-16 | 85.33 | 44.4 | 280 |
t 1877 | 3 | 07-23-12-34-12-12-23 | 84.38 | 44.4 | 290 |
t 1816 | 1 | 07-12-21-17-34-13-34-34-34-33-34 | 84.57 | 39.4 | 400 |
t 018 | 1 | 15-12-16-02-16-02-25-17-24-24-24 | 85.1 | 44.8 | 264 |
t 081 | 1 | 04-21-12-41-20-17-12-17 | 85.3 | 46.3 | 192 |