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Fig. 1 | BMC Research Notes

Fig. 1

From: Multiplex PCR for identification of two butterfly sister species: Eurema mandarina and Eurema hecabe

Fig. 1

Species-specific amplification using multiplex PCR. Lanes with odd numbers are those amplified using a universal Tpi primer set (Tpi-F/Tpi-R) and lanes with even numbers are those amplified using multiplex Tpi primer sets (Em4-F/Em4-R and Eh6-F/Eh6-R). Lanes 1–6 are E. hecabe, lanes 7–8 are E. blanda, lanes 9–20 are E. mandarina, and lanes 21–22 are a negative control. L: 100-bp ladder. OK: Okinawa Island, IS: Ishigaki Island, TW: Taiwan, IW: Iwate, NG: Nigata, MD: Matsudo, KC: Kochi, TN: Tanegashima Island

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