PCR step | Guinea pig | Capybara | |||
---|---|---|---|---|---|
All | CAPY1 | CAPY9 and CAPY24 | CAPY 4, CAPY6, CAPY7, and CAPY10 | CAPY12 | |
Initial denaturation | 95 °C for 5 min | 95 °C for 5 min | – | – | – |
# of cycles | 34 | 34 | – | –- | – |
Denaturation | 95 °C for 40 s | 94 °C for 30 s | – | – | |
Annealing | 55 °C for 30 s | 53 °C for 30 s | 54 °C for 30 s | 55 °C for 30 s | 57 °C for 30 s |
Elongation | 72 °C for 40 s | 72 °C for 40 s | – | – | – |
Final extension | 72 °C for 5 min | 72 °C for 30 min | – | – | – |
Maintenance | 4 °C | 4 °C | – | – | – |