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Table 2 Summary of the PCR profiles for cross-amplification of microsatellites in paca using 20 heterologous primer pairs developed for guinea pigs and capybara

From: Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance

PCR step

Guinea pig

Capybara

All

CAPY1

CAPY9 and CAPY24

CAPY 4, CAPY6, CAPY7, and CAPY10

CAPY12

Initial denaturation

95 °C for 5 min

95 °C for 5 min

# of cycles

34

34

–-

Denaturation

95 °C for 40 s

94 °C for 30 s

 

Annealing

55 °C for 30 s

53 °C for 30 s

54 °C for 30 s

55 °C for 30 s

57 °C for 30 s

Elongation

72 °C for 40 s

72 °C for 40 s

Final extension

72 °C for 5 min

72 °C for 30 min

Maintenance

4 °C

4 °C

  1. All guinea pig primers n = 12 (CUY5, CUY6, CUY7, CUY8, CUY9, CUY10, CUY17, CUY18, CUY22, CAVY2, CAVY11, and CAVY12; Aviles et al. [24] had the same PCR protocol. While capybara primers n = 8 (CAPY1, CAPY4, CAPY6, CAPY7, CAPY9, CAPY10, CAPY12 and CAPY24; Herrera et al. [26]) had similar conditions across the majority of the PCR profile, indicated by “–” in the table, they varied in annealing temperature