From: Chromosome segregation in B. subtilis is highly heterogeneous
Slow growth (25 °C) | Fast growth (37 °C) | |||
---|---|---|---|---|
Foci/cell | nt | Foci/cell | nt | |
PG26 (origin tag) | 1.8 ± 0.51 | 620 | 4.42 ± 1.49 | 848 |
AT62 (terminus tag) | 1.49 ± 0.6 | 700 | 1.89 ± 0.66 | 848 |
PG26 | Control 25 °C | 1 h 37 °C | 1.5 h 37 °C | 2 h 37 °C | 2.5 h 37 °C | 3 h 37 °C | |
---|---|---|---|---|---|---|---|
Percent polyploidy and average number of origins over time | |||||||
OD | 0.7 | 0.11 | 0.3 | 0.7 | 1.8 | 2.5 | |
% monoploida | 37.1 | 11 | 9 | 11 | 10 | 13 | |
% diploidb | 59.2 | 70 | 71 | 70 | 68 | 68 | |
% polyploidc | 3.7 | 19 | 20 | 19 | 22 | 19 | |
Origins per cell | 1.8 ± 0.51 | 4.8 ± 1.6 | 4.9 ± 2 | 4.9 ± 1.1 | 4.7 ± 0.55 | 4.1 ± 0.89 | |
nt | 620 | 300 | 300 | 350 | 400 | 400 |
AT62 | Control 25 °C | 1 h 37 °C | 1.5 h 37 °C | 2 h 37 °C | 2.5 h 37 °C | 3 h 37 °C | |
---|---|---|---|---|---|---|---|
Percent ploididity and average number of origins over time | |||||||
OD | 0.7 | 0.13 | 0.4 | 1 | 1.9 | 3 | |
% monoploidd | 35 | 17 | 13 | 12 | 15 | 12 | |
% diploide | 61 | 74 | 72 | 71 | 69 | 75 | |
% polyploidf | 4 | 9 | 15 | 17 | 16 | 13 | |
Termini per cell | 1.49 ± 0.6 | 1.7 ± 0.5 | 1.93 ± 0.6 | 1.8 ± 1.1 | 1.72 ± 0.73 | 1.95 ± 0.8 | |
nt | 700 | 200 | 300 | 300 | 400 | 400 |
AT62 | Control 25 °C | 1.5 h 37 °C | 2 h 37 °C | 2.5 h 37 °C |
---|---|---|---|---|
Percent ploididity from automatically calculated average DAPI fluorescence | ||||
OD | 0.7 | 0.4 | 1 | 1.9 |
% monoploid | 27.8 | 11.7 | 15 | 11.5 |
% diploid | 67.5 | 68.3 | 63.3 | 62.25 |
% polyploid | 4.6 | 20 | 21.7 | 26.25 |
nt | 540 | 300 | 300 | 400 |