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Table 1 Origins and termini count under slow and fast growth

From: Chromosome segregation in B. subtilis is highly heterogeneous

 

Slow growth (25 °C)

Fast growth (37 °C)

 

Foci/cell

nt

Foci/cell

nt

PG26 (origin tag)

1.8 ± 0.51

620

4.42 ± 1.49

848

AT62 (terminus tag)

1.49 ± 0.6

700

1.89 ± 0.66

848

PG26

Control 25 °C

1 h

37 °C

1.5 h

37 °C

2 h

37 °C

2.5 h

37 °C

3 h

37 °C

Percent polyploidy and average number of origins over time

 OD

0.7

0.11

0.3

0.7

1.8

2.5

 % monoploida

37.1

11

9

11

10

13

 % diploidb

59.2

70

71

70

68

68

 % polyploidc

3.7

19

20

19

22

19

 Origins per cell

1.8 ± 0.51

4.8 ± 1.6

4.9 ± 2

4.9 ± 1.1

4.7 ± 0.55

4.1 ± 0.89

 nt

620

300

300

350

400

400

AT62

Control

25 °C

1 h

37 °C

1.5 h

37 °C

2 h

37 °C

2.5 h

37 °C

3 h

37 °C

Percent ploididity and average number of origins over time

 OD

0.7

0.13

0.4

1

1.9

3

 % monoploidd

35

17

13

12

15

12

 % diploide

61

74

72

71

69

75

 % polyploidf

4

9

15

17

16

13

 Termini per cell

1.49 ± 0.6

1.7 ± 0.5

1.93 ± 0.6

1.8 ± 1.1

1.72 ± 0.73

1.95 ± 0.8

 nt

700

200

300

300

400

400

AT62

Control

25 °C

1.5 h

37 °C

2 h

37 °C

2.5 h

37 °C

Percent ploididity from automatically calculated average DAPI fluorescence

 OD

0.7

0.4

1

1.9

 % monoploid

27.8

11.7

15

11.5

 % diploid

67.5

68.3

63.3

62.25

 % polyploid

4.6

20

21.7

26.25

 nt

540

300

300

400

  1. % polyploidy calculated over all time points at 37 °C = 23.65%.
  2. *nt refers to the total number of cells counted in each experiment
  3. a1 or 2 origins
  4. b2 to 4 origins
  5. c4 to 8 origins
  6. *nt refers to the total number of cells counted in each time interval.
  7. d1 terminus
  8. e2 termini
  9. f> 2 termini