Table 1 Different methods described for generation of AqE
From: In vitro biological assessment of the stability of cigarette smoke aqueous aerosol extracts
Smoking regime | AqE generation parameters | Media conditions | Study |
|---|---|---|---|
HCI | 30 puffs/15 ml | Ice-cold DMEM | Munakata et al. [10] |
Other | 1 cig/10 ml | Preheated RPMI | Stabile et al. [12] |
ISO | 8 puffs/20 ml | Sterile PRF medium 199 (stored at – 80 °C) | Brunssen et al. [13] |
NS | 10 cigs/10 ml | Solvent (unspecified) | Ji et al. [14] |
HCI | 10 puffs/20 ml | VascuLife basal media† with supplements | Taylor et al. [7] |
ISO | 8 puffs/20 ml | PRF DMEM/F12 | Oke et al. [15] |
NS | 8–10 puffs/35 ml | Saline solution | Gellner et al. [16] |
NS | Smoke/25 ml | Media (unspecified) | Comer et al. [17] |
NS | 1 cig/10 ml | PBS | Yoon et al. [18] |
NS | 1 cig/10 ml | DMEM (Stored at –32 °C) | Streck et al.; Kim et al. 2002 [19] |
NS | 1 cig/10 ml | PBS | Nana-Sinkam et al. [20] |
NS | 1 cig/25 ml | RPMI-1640 (one cigarette without filter) | Kim et al. [21] |
NS | 2 cig/50 ml | RPMI-1640 (filters removed from two 1R4F cigarettes) | Richter et al. [22] |