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Fig. 3 | BMC Research Notes

Fig. 3

From: Enhancement of intestinal epithelial barrier function by Weissella confusa F213 and Lactobacillus rhamnosus FBB81 probiotic candidates in an in vitro model of hydrogen peroxide-induced inflammatory bowel disease

Fig. 3

Single strain (WCF213 or LrFBB81) and combination strains (WCF213 and LrFBB81) pretreatment helped in maintaining mucosal integrity against H2O2 exposure. Caco-2 cells were untreated (control; a), treated with H2O2 only (b) and pretreated with probiotics strains (WCF213 (c), LrFBB81 (d), and combination (e) for 2 h, 37 °C, 5% CO2 before TJ disruption with 25 mM H2O2 for 4 h. ZO-1 tight junction protein was detected using immunofluorescence. ZO-1 protein of Caco-2 cells pretreated with probiotics strains (c, d, e) was maintained much better than that treated only with H2O2 (b). Cells that treated with hydrogen peroxide showed loss of their tight junction as shown as arrowheads (b) (observation 60× oil immersion, 10 microscope field of each treatment)

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BMC Research Notes

ISSN: 1756-0500

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