Fig. 3

JARID2-AS1 is enriched in the nucleus, it interacts with PRC2 and might be involved in auto-regulation of JARID2 a Agarose gel showing untreated and treated RNA with DNase I to remove genomic DNA from nuclear (NF) and cytosolic (CF) fractions. Ribosomal RNA bands are seen only in CF. b JARID2-AS1 is enriched in the nucleus in both undifferentiated (D0) and differentiated (D6) HaCaTs. c qRT-PCR data also shows JARID2-AS1 is significantly enriched in the nucleus. U105 snoRNA was used as nuclear control, whereas actin was used as equal loading control. Relative expression (n = 3) was normalized to actin and analysed using the student’s t test (** P value < 0.01). d RNA–protein binding data (accession number GSE36070) was analysed and showed that JARID2-AS1 is enriched in EZH2 bound RNAs. MALAT1 was previously shown to interact with EZH2 in many cancers, therefore, it is used as positive control. e A model showing autoregulation of JARID2. JARID2 protein is a known regulatory partner of PRC2 complex. We hypothesize that JARID2-AS1 might negatively regulate the transcription of JARID2 isoform-1 by interacting with EZH2 and JARID2 thus forming an auto-regulatory loop