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Table 3 PCR condition for bcsp31 template

From: Comparison of loop-mediated isothermal amplification and conventional PCR tests for diagnosis of common Brucella species

Feature

Temperature (°C)

Time

Gene (bcsp31)

 Initial denaturation

95

5 min

 Denaturation

95

60 s

 Annealing

65

30 s

 Extension

72

60 s

 Final extension

72

6 min

 Cycle

35

–

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BMC Research Notes

ISSN: 1756-0500

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