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Fig. 1 | BMC Research Notes

Fig. 1

From: Activity of the mouse Notch ligand DLL1 is sensitive to C-terminal tagging in vivo

Fig. 1

Analysis of DLL1AcGFPHA and DLL1SF proteins in CHO cells. A Scheme of constructs used to express DLL1AcGFPHA or DLL1SF in CHO cells under control of the CMV2 promoter. Full length construct (top), 3’ region before (middle), and after (bottom) Cre-mediated recombination. B Expression of DLL1 variants in CHO cells carrying DLL1AcGFPHA or DLL1SF. DLL1 variants were immunoprecipitated with anti HA (a) anti-GFP (b) or anti-Flag (c) antibodies and detected in Western blots using anti-DLL1 antibody 1F9. + control: Lysate of CHO cells stably overexpressing DLL1. Arrows point to tagged DLL1 proteins. Photographs of the Western blot membranes are shown in Additional file 7: Figure S3. C AcGFP fluorescence in DLL1AcGFPHA expressing CHO cells (row a) in comparison to wild-type CHO cells (row b); Column I: fluorescence, II: overlay, III: bright field. D Surface presentation of DLL1AcGFPHA (dots) and DLL1SF (triangles) in CHO detected in cell surface biotinylation assays

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