Fig. 2

Mutation rate (a) and mutation efficiency (b) of blastocysts derived from zona pellucida (ZP)-free zygotes subjected to lipofection-mediated gene editing with gRNA targeting GGTA1. Zygotes were collected at 10 h from the start of in vitro fertilization and incubated with Lipofectamine 2000, gRNA, and Cas9 for 2.5 h, 5 h, 10 h, or 20 h. The mutation rate was defined as the ratio of the number of edited blastocysts to the total number of sequenced blastocysts. Mutation efficiency was estimated as the proportion of the indel mutation events in gene-edited blastocysts. Numbers within parentheses indicate the total number of blastocysts examined. Each bar represents the mean ± SEM. *p < 0.05