A genetic linkage map of Venturia inaequalis, the causal agent of apple scab

BMC Research Notes

Table 1 AFLP primer pairs used for map construction

AFLP primer pairs		Polymorphic bands			Markers with distorted segregation from 1:1^a			Mapped markers
Code	Composition	Q	W	Total	P 0.05	P < 0.01	P < 0.001	Q	W	Total
F	M^b+C/E^c+AG^d	17	13	30	11	11	9	9	10	19
H	M+C/E+AT	18	24	42	20	19	12	9	10	19
K	M+C/E+TG	19	17	36	15	11	10	13	10	23
L	M+G/E+TG	18	19	37	27	26	22	3	8	11
M	M+G/E+AG	7	11	18	11	8	6	4	10	14
N	M+G/E+AT	25*** ^e	5	30	17	13	10	6	4	10
O	M+A/E+AG	15	8	23	16	15	14	4	6	10
P	M+A/E+AT	14*	4	18	13	13	8	11	4	15
R	M+T/E+AG	17	17	34	15	13	11	8	10	18
S	M+T/E+AT	21	21	42	24	18	15	14	13	27
T	M+C/E+AA	8	8	16	12	12	9	1	4	5
V	M+C/E+AC	16	14	30	18	9	6	10	13	23
W	M+A/E+TG	8	5	13	9	8	7	4	0	4
X	M+T/E+TG	14	19	33	18	16	12	8	10	18
Y	M+A/E+AA	8	12	20	15	13	11	5	5	10
Z	M+T/E+AA	23	12	35	20	19	16	9	7	16
AA	M+A/E+AC	16	14	30	16	13	13	12	12	24
BB	M+T/E+AC	16	15	31	18	14	9	9	8	17
	Total	280	238	518	295	251	200	139	144	283

Table 1 lists the AFLP primer pairs used for screening apple scab isolates, and segregation and mapping data for AFLP markers generated for the construction of the genetic linkage map of Venturia inaequalis
^a: Number of markers with a segregation ratio deviating from the 1:1 ratio based on the exact probability from an one sample binomial test
^b: M stands for MseI sequence – GATGAGTCCTGAGTAA (5' to 3')
^c: E stands for EcoR1 sequence – AGACTGCGTACCAATTC (5' to 3')
^d: FAM was attached to E + AA, E + AC and E + AT; VIC to E + AG and NED to E + TG
^e: *, *** indicates that the C0154 isolate contributed significantly more alleles to the progeny than the 01/213 isolate at the level of P = 0.05 and 0.001, respectively

ISSN: 1756-0500