Table 1 Expression values of 6 reference genes using qPCR in purified versus non-purified pre-amplified samples
From: RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts
UBC | GAPDH | RPL13A | YWHAZ | SDHA | HPRT1 | mean dCq | 2^dCq | ||
|---|---|---|---|---|---|---|---|---|---|
sample 1 | mean Cq NP1 (n = 2) | 25.47 | 23.73 | 19.05 | 22.31 | 29.82 | 25.71 | ||
mean Cq P1 (n = 2) | 21.54 | 22.19 | 15.87 | 18.29 | 25.94 | 22.03 | |||
dCq | 3.93 | 1.54 | 3.18 | 4.02 | 3.88 | 3.68 | 3.37 | 10.34 | |
sample 2 | mean Cq NP2 (n = 2) | 23.71 | 23.03 | 18.12 | 21.81 | 30.14 | 24.86 | ||
mean Cq P2 (n = 2) | 20.95 | 21.54 | 15.63 | 18.53 | 28.14 | 21.89 | |||
dCq | 2.77 | 1.49 | 2.49 | 3.28 | 2.01 | 2.97 | 2.5 | 5.65 | |
sample 3 | mean Cq NP3 (n = 2) | 24.22 | 23.03 | 18.63 | 20.94 | 30.17 | 25.82 | ||
mean Cq P3 (n = 2) | 21.1 | 21.75 | 16.04 | 17.66 | 28.67 | 22.72 | |||
dCq | 3.12 | 1.29 | 2.6 | 3.28 | 1.5 | 3.1 | 2.48 | 5.57 | |
sample 4 | mean Cq NP4 (n = 2) | 22.96 | 23.08 | 18.54 | 21.74 | 30.66 | 25.62 | ||
mean Cq P4 (n = 2) | 19.69 | 21.89 | 16.07 | 18.63 | 28.83 | 22.48 | |||
dCq | 3.27 | 1.19 | 2.48 | 3.11 | 1.83 | 3.14 | 2.5 | 5.66 | |
sample 5 | mean Cq NP5 (n = 2) | 23.21 | 22.72 | 17.96 | 20.84 | 28.17 | 25.37 | ||
mean Cq P5 (n = 2) | 20.64 | 21.22 | 15.6 | 17.83 | 25.73 | 22.11 | |||
dCq | 2.57 | 1.51 | 2.36 | 3.01 | 2.44 | 3.26 | 2.52 | 5.74 | |
sample 6 | mean Cq NP6 (n = 2) | 24.62 | 23.48 | 18.56 | 22.22 | 28.84 | 25.71 | ||
mean Cq P6 (n = 2) | 21.52 | 21.87 | 15.91 | 19.03 | 26.97 | 22.67 | |||
dCq | 3.1 | 1.61 | 2.65 | 3.19 | 1.88 | 3.04 | 2.58 | 5.96 | |
average | 6.31 (95% CI: 4.89 - 8.14) |