Figure 3

Analysis of predicted interactive zones of Shp-2 and c-Kit. A. Distances between interactive zones of both Shp-2 and Kit kinase based on different repulsion values. Shp-2 bound Kit kinase complexes are generated using different repulsion values from 5.8A° to 6.6 A°. Distance measured between Asp816 of Kit activation loop and Tyr100 of N-sh2 domain (as Tyr100 has been found to be prevalently closer to activation loop in all models). Among the 1-6 complexes, only 6.4A° and 6.6 A° based models have activation loop residue Asp816 closest to N-sh2 domain. B. Analysis of the catalytic cleft and the residues involved. Residues such as Cys788, Lys818 and Asn819 (orange), Thr916 and Phe 848 (blue), Thr847 and His790 (green), Asn787, Asp786 and Lys 918 (white), Lys786, Leu 912 and Tyr646 (mauve color), contribute in making a catalytic cleft like structure in c-Kit.