Figure 5

PCR amplification for distinguishing null alleles of the waxy genes and wild type in wheat. (A) PCR results with primer set Wx-7A-F1 and Wx-7A-R1a to distinguish the wild type (Wx-A1a) and null allele (Wx-A1b) of the Wx-A1 gene. (B) PCR results with primer set Wx-7B-F2 and Wx-7B-R2 to distinguish the wild type (Wx-B1a) and null allele (Wx-B1b) of the Wx-B1 gene. (C) PCR results with primer set Wx-7D-F3 and Wx-7D-R3a to distinguish the wild type (Wx-D1a) and null allele (Wx-D1b) of the Wx-D1 gene. M = 1 kb plus DNA ladder. Lanes: 1, CS; 2, Sturdy; 3, Fujimi Komugi; 4, Gabo; 5, Gamenya; 6, Santanta; 7, Bai Huo; 8, Kanto 107; 9, NSGC 8645; 10, NSGC 8646.