Figure 5

RhoA does not affect flow-mediated activation of ENaC. (A, B) Summary graph of an extent (A) and half-time (B) ENaC inhibition after switching off a perfusion. CHO cells expressing either mENaC alone or coexpressed with constitutively active (G14V) or dominant negative (T19N) RhoA in the absence and presence of treatment with Y27632 (1 μM; 1 hr). The number of observations for each group is shown. All other conditions are the same as in Figure 3.