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Figure 2 | BMC Research Notes

Figure 2

From: FONZIE: An optimized pipeline for minisatellite marker discovery and primer design from large sequence data sets

Figure 2

The FONZIE workflow: The FONZIE workflow consists of 6 steps. a Execution of TRF on a Fasta sequence or a Multifasta file, b Optional: exclusion of some user-defined specific regions, c Screening by core motif size and percent match parameters, d Screening for marker specificity with BLAST (1e-10 by default) against a database, e Primer design step using PRIMER3, f Virtual PCR by making a BLAST (1e-40 by default) against the database to check the specificity of the PCR product (single copy locus).

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BMC Research Notes

ISSN: 1756-0500

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