Figure 5

Effect of siRNA on mRNA measurements when added to various steps of real-time RT-qPCR. (A) The siRNAs RRM1-2, RRM1-3 and ApoB1 were added at the indicated concentration (μM) to the cDNA template prior to real-time qPCR using primers flanking the RRM1-2 site (RRM1-B). (B) The siRNA, anstisense or sense strands of RRM1-3, or RRM1-2 siRNA were added at the indicated concentration (μM) to the RNA isolated from the tumour of a PBS-treated mouse prior to reverse transcription. (C) RRM1-2 or RRM1-3 siRNA (25 μg) or water was added to tumours before RNA isolation and RRM1 mRNA was quantified using primer sets RRM1-A or RRM1-B.