The profile of total anti-Hsp60 IgG antibody response after DNA vaccination in mice. a) IgG antibody titer; b) IgG1 isotype titer; c) IgG2a isotype titer. Groups of mice were immunized with the DNA vaccine as follows: intramuscular injection of vector pVAX1 and recombinant vector pVAX1/hsp60. Data are expressed as mean ± S.D. values. Results are representative of n = 5. Statistically significant differences between pVAX1/hsp60 and pVAX1 immunized mice are denoted by an asterisk (p < 0.05).