Table 1 Candidate reference genes evaluated in this study
Gene Symbol | Gene Name | Genbank Acession No. | TaqMan®Assay ID | Amplicon length |
|---|---|---|---|---|
18S | Eukaryotic 18S rRNA | X03205.1 (mRNA) | Hs99999901_s1 | 187 |
ABL1 | v-abl Abelson murine leukemia oncogene homolog 1 | NM_005157.3 + NM_007313.2 | Hs00245445_m1 | 91 |
ACTB | Actin, Beta, cytoplasmic | NM_001101.3 | Hs99999903_m1 | 171 |
B2M | Beta-2-microglobulin | NM_004048.2 | Hs99999907_m1 | 75 |
CASC3 | cancer susceptibility candidate 3 | NM_007359.4 | Hs00201226_m1 | 67 |
CDKN1A | cyclin-dependent kinase inhibitor 1A (p21, Cip1) | NM_078467.1 + NM_000389.3 | Hs00355782_m1 | 66 |
CDKN1B | cyclin-dependent kinase inhibitor 1B (p27, Kip1) | NM_004064.3 | Hs00153277_m1 | 71 |
EIF2B1 | eukaryotic translation initiation factor 2B, subunit 1 alpha, 26 kDa | NM_001414.3 | Hs00426752_m1 | 75 |
ELF1 | E74-like factor 1/ets domain transcription factor) | NM_172373.3 + NM_001145353.1 | Hs00152844_m1 | 76 |
GADD45A | growth arrest and DNA-damage-inducible, alpha | NM_001924.2 | Hs00169255_m1 | 123 |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | NM_002046.3 | Hs99999905_m1 | 122 |
GUSB | Glucuronidase, Beta | NM_000181.3 | Hs99999908_m1 | 81 |
HMBS | Hydromethylbilane synthase | NM_000190.3 | Hs00609297_m1 | 64 |
HPRT1 | Hypoxanthine guanine phospho- ribosyl transferase 1 | NM_000194.2 | Hs99999909_m1 | 100 |
IPO8 | Improtin 8 | NM_006390.2 | Hs00183553_m1 | 71 |
MRPL19 | mitochondrial ribosomal protein L19 | NM_014763.3 | Hs00608519_m1 | 72 |
MT-ATP6 | mitochondrially encoded ATP synthase 6 | NC_001807.ATP6.0 | Hs02596862_g1 | 150 |
PES1 | pescadillo homolog 1, containing BRCT domain (zebrafish) | NM_014303.2 | Hs00362795_g1 | 56 |
PGK1 | Phosphoglycerate kinase 1 | NM_000291.3 | Hs99999906_m1 | 75 |
POLR2A | Polymerase (RNA) II (DNA directed) polypeptide A, 220 kDa | NM_000937.3 | Hs00172181_m1 | 61 |
POP4 | processing of precursor 4, ribonuclease P/MRP subunit | NM_006627.2 | Hs00198357_m1 | 68 |
PPIA | Peptidylprolyl Isomerase A | NM_021130.3 | Hs99999904_m1 | 98 |
PSMC4 | proteasome (prosome, macropain) 26S subunit, ATPase, 4 | NM_153001.1 + NM_006503.2 | Hs00197826_m1 | 83 |
PUM1 | pumilio homolog 1 (Drosophila) | NM_001020658.1 + NM_014676.2 | Hs00206469_m1 | 89 |
RPL30 | ribosomal protein L30 | NM_000989.2 | Hs00265497_m1 | 149 |
RPL37A | ribosomal protein L37A | NM_000998.4 | Hs01102345_m1 | 125 |
RPLP0 | Ribosomal protein, large, P0 | NM_053275.3 + NM_001002.3 | Hs99999902_m1 | 105 |
RPS17 | ribosomal protein S17 | NM_001021.3 | Hs00734303_g1 | 93 |
TBP | TATA binding box protein | NM_008907 | Hs99999910_m1 | 127 |
TFRC | Transferrin receptor | NM_001128148.1 + NM_003234.2 | Hs99999911_m1 | 105 |
UBC | Ubiquitin C | NM_021009.4 | Hs00824723_m1 | 71 |
YWHAZ | Tyrosine 3-monooxygenase | NM_003406.3 | Hs00237047_m1 | 70 |