Figure 2

Immunofluorescenence analysis of CD99 expression on Ramos wild type and CD99 sorted cells. Ramos cells were chosen for establishment of CD99 expressing and non-expressing cells by an immunomagnetic sorting system using anti-CD99 mAb. The sorted cells were then verified for their surface CD99 expression by immunoflurorescence and flow cytometry. The wild type (A), CD99 positive (B) and CD99 negative (C) cells were stained with anti-CD99 mAbs MT99/3 and PE-conjugated anti-mouse immunoglobulin antibodies. Solid lines represent the immunofluorescence profiles of the cells stained with anti-CD99 mAb and dashed lines represent background fluorescence of conjugate control.