Table 1 Gene specific-primers and key PCR conditions.
Gene Symbol(GenBank #) Name | Primer (5'-3')* | Anneal-ing Temp (°C) | Ampli-con Size (bp) | PCR Efficiency |
|---|---|---|---|---|
YWHAZ (NM_003406) Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide | FW: TGATCCCCAATGCTTC RV: TGTTGTGACTGATCGAC | 60 | 127 | 1.81 |
PPIB (NM_000942) Peptidylprolyl isomerase B | FW: CAGCAAATTCCATCGTG RV: CCGTAGTGCTTCAGTTT | 60 | 132 | 1.97 |
RPLP0 (NM_015741) Ribosomal protein, large, P0 | FW: CCTTCTCCTTTGGGC RV: CCGTAGTGCTTCAGTTT | 60 | 133 | 1.83 |
PGK1 (NM_000291) Phosphoglycerate kinase 1 | FW: CAAGAAGTATGCTGAGGCTGTCA RV: CAAATACCCCCACAGGACCAT | 58 | 68 | 1.93 |
POLR2A (NM_000937) Polymerase (RNA) II (DNA directed) polypeptide A, 220 kDa | FW: ATCTCTCCTGCCATGACACC RV: AGACCAGGCAGGGGAGTAAC | 62 | 162 | 1.94 |
EAR Expressed Alu Repeats | FW: GAGGCTGAGGCAGGAGAATCG RV: GTCGCCCAGGCTGGAGTG | 62 | 87 | 1.91 |
CAB (X56062) Arabidopsis thaliana chlorophyll A-B binding protein | FW: CTCAGGAATGGGCAGCACTACC RV: CAGAATCCTACAAACGCCAACAGC | 60 | 273 | 1.96 |