Table 5 PCR cycling conditions
Primers | Initial Denaturation | Denaturation | Annealing Elongation | Cycles | Terminal Elongation |
|---|---|---|---|---|---|
KanR F and ITSR-Eub R | 95°C/5 min | 95°C/30 s | 54°C/25 s 72°C/70 s | 35 | 72°C/7 min |
16S-926 F and GFP R: | 95°C/5 min | 95°C/30 s | 53°C/40 s 72°C/90 s | 35 | 72°C/7 min |
16S-Pr.mir-530 F and GFP F | 95°C/5 min | 95°C/30 s | 63°C/25 s 72°C/70 s | 35 | 72°C/7 min |
16S | 94°C/15 min | 94°C/4 min | 50°C/45 s 72°C/1 min | 5 |  |
16S (cont.) |  | 92°C/45 s | 55°C/45 s 72°C/1 min | 30 | 72°C/7 min |
13 F-14 F | 97°C/5 min | 97°C/30 s | 50°C/45 s 72°C/1.5 min | 30 | 72°C/7 min |
16 F-17 F | 97°C/5 min | 97°C/30 s | 50°C/45 s 72°C/1 min | 30 | 72°C/7 min |
E9F-K1 | 97°C/5 min | 97°C/30 s | 56°C/45 s 72°C/1.5 min | 30 | 72°C/7 min |
Kmup-97ar | 97°C/5 min | 97°C/30 s | 56°C/45 s 72°C/2.5 min | 30 | 72°C/7 min |