Dataset | Random | Unique | BowStrap |
---|
50 M
| 0.91 | 0.80 | 1.00 |
25 M
| 0.90 | 0.80 | 1.00 |
10 M
| 0.90 | 0.81 | 0.99 |
1 M
| 0.89 | 0.81 | 0.96 |
0.5 M
| 0.87 | 0.81 | 0.94 |
- Spearman’s Rank Correlation was calculated between gene model expression values in synthetic read data and ‘Bowtie’-based methods for estimation of gene model expression levels. Three ‘Bowtie’-based methods for gene model expression were considered, using gene model expression values from synthetic RNAseq data of 50, 25, 10, 1, and 0.5 million total 46-mer sequence reads.