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Table 3 Pairwise correlation analyses using BestKeeper

From: Evaluation of qPCR reference genes in two genotypes of Populus for use in photoperiod and low-temperature studies

Populus trichocarpa           
Shoot tips/buds Coeff. of corr. (r) p-value Young leaves Coeff. of corr. (r) p-value Mature leaves Coeff. of corr. (r) p-value Bark Coeff. of corr. (r) p-value
UBQ7 0.892 0.001 CDC2 0.970 0.001 18S rRNA 0.964 0.001 CDC2 0.993 0.001
18S rRNA 0.810 0.001 ANT 0.963 0.001 ACT2 0.962 0.001 PT1 0.993 0.001
CDC2 0.718 0.003 ACT2 0.961 0.001 CDC2 0.943 0.001 ACT2 0.983 0.001
PT1 0.660 0.007 18S rRNA 0.799 0.001 ANT 0.917 0.001 UBQ7 0.980 0.001
ACT2 0.655 0.008 PT1 0.610 0.016 UBQ7 0.845 0.001 ANT 0.970 0.001
CYC063 0.639 0.010 UBQ7 0.532 0.041 PT1 0.663 0.007 CYC063 0.969 0.001
ANT 0.360 0.188 TIP4-like 0.517 0.048 TIP4-like 0.088 0.754 TIP4-like 0.957 0.001
TIP4-like 0.223 0.427 CYC063 −0.008 0.977 CYC063 −0.477 0.072 18S rRNA 0.650 0.009
Populus tremula x Populus alba          
Shoot tips/buds Coeff. of corr. ( r ) p -value Young leaves Coeff. of corr. ( r ) p -value Mature leaves Coeff. of corr. ( r ) p -value Bark Coeff. of corr. ( r ) p -value
CDC2 0.905 0.001 CDC2 0.977 0.001 ACT2 0.894 0.001 PT1 0.812 0.001
ACT2 0.862 0.001 ANT 0.944 0.001 18S rRNA 0.816 0.001 CDC2 0.793 0.001
ANT 0.788 0.001 18S rRNA 0.886 0.001 ANT 0.653 0.008 ANT 0.738 0.002
18S rRNA 0.659 0.008 ACT2 0.881 0.001 CDC2 0.569 0.027 ACT2 0.650 0.009
UBQ7 0.651 0.009 PT1 0.562 0.029 CYC063 0.420 0.119 CYC063 0.647 0.009
PT1 0.562 0.029 TIP4-like 0.416 0.123 UBQ7 0.323 0.240 UBQ7 0.644 0.009
TIP4-like 0.457 0.087 UBQ7 0.358 0.191 TIP4-like 0.057 0.837 TIP4-like 0.633 0.011
CYC063 0.129 0.646 CYC063 −0.717 0.003 PT1 0.050 0.860 18S rRNA 0.389 0.152
  1. Each candidate reference gene was compared to the BestKeeper Index to calculate a Pearson’s coefficient of correlation (r) and a p-value. Larger correlation coefficients indicate greater correlation with the index and is evidence of higher gene expression stability.