Figure 1
From: Seed storage proteins of the globulin family are cleaved post-translationally in wheat embryos
SDS-PAGE and immunoblot analysis of AC Barrie salt-soluble proteins. The salt-soluble fraction from AC Barrie seeds was separated under reducing conditions by SDS-PAGE (12% polyacrylamide) and stained with CBB R-250. Standard lane (M) is Precision Plus Protein (Bio-Rad). Proteins were immunoblotted with polyclonal anti-Glo-3A antiserum at a 1:10,000 dilution, with pre-immune serum (1:10,000), or with secondary and tertiary antibodies alone.