Skip to main content

Table 1 Comparison of blood samples collected in PAXgene and in Tempus tubes

From: Human blood RNA stabilization in samples collected and transported for a large biobank

A) Adult blood

Storage at RT (days)

RIN value

OD 260/280 ratio

OD 260/230 ratio

Number of tubes

PAXgenes tube

0

8.0 ± 0.3

2.12 ± 0.02

1.56 ± 0.31

5

2

8.0 ± 0.3

2.10 ± 0.03

1.56 ± 0.62

5

5

8.2 ± 0.3

2.12 ± 0.02

1.37 ± 0.73

5

7

8.2 ± 0.3

2.09 ± 0.03

1.42 ± 0.70

5

Average

8.10 ± 0.3

2.11 ± 0.02

1.48 ± 0.61*

20

Tempus tubes

0

8.0 ± 0.3

2.12 ± 0.07

2.01 ± 0.14

5

2

8.2 ± 0.3

2.12 ± 0.07

2.01 ± 0.14

5

5

8.0 ± 0.3

2.14 ± 0.08

2.09 ± 0.08

5

7

8.2 ± 0.3

2.11 ± 0.04

2.08 ± 0.06

5

Average

8.10 ± 0.3

2.12 ± 0.07

2.05 ± 0.11

20

B) Cord blood

 

PAXgene (n = 8)

 

Tempus (n = 7)

 

RIN value

8.1 ± 0.2

 

8.0 ± 0.2

 

OD 260/280 ratio

2.1 ± 0.1

 

2.0 ± 0.3

 

OD 260/230 ratio

1.6 ± 0.5*

 

2.0 ± 0.8

 
  1. Notes. * RNA from PAXgene tube-collected samples have significantly lower OD 260/230 ratio compared to RNA from Tempus tube-collected samples, p < 0.05. The cord blood samples were transported to NIPH (lab) within one day. n = number of tubes.
Back to article page

BMC Research Notes

ISSN: 1756-0500

Contact us