Figure 2

Gene expression of IFN-γ. Relative gene expression of IFN-γ in chicken CD4+ T cells at 1, 3, 8 and 18 hours post-treatment with low (1 μg/mL) and high (5 μg/mL) doses of the TLR ligands Pam3CSK4, LPS and CpG ODN, or with a low (10 μg/mL) or high (50 μg/mL) dose of poly I: C. Graphed data represent mean fold change of 4 treatment replicates compared to the medium control group ± standard error. Results were considered statistically significant from the medium-treated control group if p ≤ 0.05 (*) and p ≤ 0.01 (**).