Table 1 DNA sequences of RT-PCR, quantitative RT-PCR, and DNA sequencing primers
Target | Sense primer (location) | Antisense primer (location) |
|---|---|---|
RT-PCR and DNA sequencing | ||
NM_001175 | ACTCAGAAGTCAGAGTTGAGAGAC (exon 1) | AGAGAATTCTTCCAGGTGGCAAGG (exon 6/6x) |
variant 6 series | ACTGGGCTTTTGCTTCATTTGTTCAG (exon 1x/y/z) | AGAGAATTCTTCCAGGTGGCAAGG (exon 6/6x) |
CGACTGGAGCACGAGGACACTGA (5′ RACE) | ACGAGTGAAGCAACATGGCACAAAG (exon 1x/y/z) | |
ACTGGGCTTTTGCTTCATTTGTTCAG (exon 1x/y/z) | GCTGTCAACGATACGCTACGTAACG (3′ RACE) | |
AGGAGTCTTTGTGCCATGTTGCTTCAC (exon 1x/y/z) | CGCTACGTAACGGCATGACAGTG (3′ RACE) | |
GGACACTGACATGGACTGAAGGAGTA (5′ RACE) | ACTCCTGAACAAATGAAGCAAAAGCCCC (exon 1x/y/z) | |
ACTB | ATTCCTATGTGGGCGACGAGGC (exon 3) | TGGATAGCAACGTACATGGCTGG (exon 4) |
Quantitative RT-PCR | ||
NM_001175 | GGACAGAGACGTGAAGCACTGA (exon 1) | GCTTGCTGTCCAGCTCATCA (exon 2) |
variant 6a | GTCTTTGTGCCATGTTGCTTCA (exon 1x/y/z) | AGCTTGCTGTCCAGCTCATCA (exon 2) |
ACTB | TGGCACCCAGCACAATGA (exon 5) | CCGATCCACACGGAGTACTTG (exon 6/6x) |
Standard curves in quantitative RT-PCR | ||
NM_001175 | ACTCAGAAGTCAGAGTTGAGAGAC (exon 1) | AGAGAATTCTTCCAGGTGGCAAGG (exon 6/6x) |
variant 6a | ACTGGGCTTTTGCTTCATTTGTTCAG (exon 1x/y/z) | ACTCTCACAAACCAGGGTGAGC (exon 3) |