Validation of reference genes for quantitative real-time PCR studies in the dentate gyrus after experimental febrile seizures

Table 1 Selected reference genes for analysis of expression stability

Gene symbol	Gene function	Primer sequence (5’ → 3’)^aor SABiosciences qPCR assay ID^b	Amplicon length (bp)
ActB	Cytoskeletal structural protein	F: TGT CAC CAA CTG GGA CGA TA	165
ActB	Cytoskeletal structural protein	R: GGG GTG TTG AAG GTC TCA AA	165
CycA	Serine-threonine phosphatase inhibitor	F: TAT CTG CAC TGC CAA GAC TGA GTG	126
CycA	Serine-threonine phosphatase inhibitor	R: CTT CTT GCT GGT CTT GCC ATT CC	126
18S rRNA	Ribosomal subunit	F: ACG GAC CAG AGC GAA AGC AT	310
18S rRNA	Ribosomal subunit	R: TGT CAA TCC TGT CCG TGT CC	310
Rpl13A	Structural component of 60S ribosomal subunit	F: GGA TCC CTC CAC CCT ATG ACA	132
Rpl13A	Structural component of 60S ribosomal subunit	R: CTG GTA CTT CCA CCC GAC CTC	132
Tbp	General transcription factor	F: TGG GAT TGT ACC ACA GCT CCA	131
Tbp	General transcription factor	R: CTC ATG ATG ACT GCA GCA AAC C	131
GusB	Exoglycosidase in lysosomes	PPR43194B^b	137
Arbp	Catalysis of protein synthesis	PPR42394A^b	92
Cnr1	Endocannabinoid signalling	PPR52793A^b	156

ActB, beta-actin; CycA, CyclophilinA; 18S rRNA, 18S subunit ribosomal RNA; Rpl13A, Ribosomal protein L13A; Tbp, TATA box binding protein; GusB, beta-glucuronidase; Arbp, Acidic ribosomal phosphoprotein P0; Cnr1, Cannabinoid Type 1 (CB1) receptor. F, forward primer; R, reverse primer.
^a primer sequences are based on literature[24].
^b ID, identification.

ISSN: 1756-0500