Table 2 M. smithii real-time PCR detection in 15 non-diarrheal stool specimens diluted in 1:5 in sterile phosphate buffer, before and after lyophilization (Cycle threshold [Ct] value)
From: Optimized microbial DNA extraction from diarrheic stools
Sample n° | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Without lyophilization | 40.5 | NA | 38.93 | 36.43 | 26.38 | NA | 27.92 | 39.63 | NA | 22.27 | 33.43 | 43.61 | 38.53 | NA | 23.39 |
With lyophilization | 34.39 | 34.74 | 33.93 | 16.97 | 16.84 | 11.72 | 20.16 | 30.24 | 19.76 | 23.11 | 22.65 | NA | 31.15 | 23.28 | 22.41 |