Figure 4

Average relative fluorescence intensity for N. glauca TD-GFP-K T ₁ lines. Leaf tissue from a non-transgenic N. glauca line and eight transgenic T₁ lines were excited at 395 nm and measured at an emission of 509 nm with a spectrofluorometer. Fluoresence intensity values were normalized to an average measurement of N. glauca negative control plants outside the spectral range of GFP emission. For N. glauca n = 2, TD-GFP-K lines 1 n = 26, line 2 n = 34, line 3 n = 31, line 4 n = 16, lines 5 and 6 n = 33, line 7 n = 28, and line 8 n = 24. Error bars represent the standard error from the mean and different letters indicate significant differences at P < 0.05.