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Table 2 Reference genes ranking for the entire dataset calculated by the GeNorm, NormFinder and BestKeeper

From: Validation of reference genes for normalization of qPCR gene expression data from Coffea spp. hypocotyls inoculated with Colletotrichum kahawae

Entire dataset

Gene

GeNorm

Normfinder

Bestkeeper

M

SV

CV ± SD

r

IDE

0.578(1/2)

0.402(1)

2.54 ± 0.62(2)

0.801*

14-3-3

0.578(1/2)

0.624(4)

2.61 ± 0.59(4)

0.723*

RPL7

0.604(3)

0.542(3)

1.98 ± 0.47(1)

0.701*

β-Tub9

0.684(4)

0.478(2)

2.44 ± 0.47(3)

0.720*

S24

0.731(5)

0.610(5)

2.79 ± 0.57(5)

0.660*

UBQ9

0.785(6)

0.699(6)

2.23 ± 0.54(6)

0.560

GAPDH

1.019(7)

1.597(7)

n.a

n.a

  1. SV. stability value; CV, coefficient of variance; SD, standard deviation of Cq value; r. Pearson coefficient of correlation; * p ≤ 0.001. p-value associated with the Pearson coefficient of correlation. Ranking order is presented in parenthesis, n.a., not assigned.
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BMC Research Notes

ISSN: 1756-0500

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