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Table 6 Reference genes ranked from the most to the least stable considering healthy Euscelidius variegatus samples

From: Selection of reference genes from two leafhopper species challenged by phytoplasma infection, for gene expression studies by RT-qPCR

Ranking order

BestKeeper (N = 16)

geNorm (N = 17)

Normfinder (N = 17)

 

Gene name

Std dev [± CP]

Gene name

M value

Gene name

Stability value

1

18S

0.31

Tropomyosin

1.225

ATP Syn β

0.127

2

Tropomyosin

1.01

GAPDH *

1.296

Tropomyosin

0.130

3

GAPDH

1.06

ATP Syn β *

1.366

GAPDH

0.160

4

Actin

1.14

18S

1.482

Actin

0.170

5

ATP Syn β

1.46

Actin

1.611

18S

0.176

  1. *Best combination of two genes according with geNorm (average expression stability M = 0.823).
  2. Best combination of two genes according with Normfinder (stability value = 0.074).
  3. In bold geNorm M values > 1.5.
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BMC Research Notes

ISSN: 1756-0500

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