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Figure 1 | BMC Research Notes

Figure 1

From: Evaluation of DNA extraction from granulocytes discarded in the separation medium after isolation of peripheral blood mononuclear cells and plasma from whole blood

Figure 1

Diagrammatic representation of granulocytes in separation medium evaluated for DNA extraction in this study. After centrifugation of diluted whole blood over-layed on Ficoll medium, usually plasma (layer 1) and white blood cells (layer 2) are recovered for further studies. The residual whole blood containing ficoll solution (layer 3) and erythrocytes/granulocytes (layer 4), which is often discarded, was evaluated in this study as a rich source of high quality DNA without further purifications of cells from separation medium.

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BMC Research Notes

ISSN: 1756-0500

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