Figure 4

CBSD symptoms on leaves, stems and storage roots as transmitted by chip bud grafts carrying CBSV. (A) chlorotic spots seen as first evidence of successful transmission on a leaf of cultivar Ebwanateraka, (B) feathery chlorosis on veinal regions of a leaf of cultivar Ebwanateraka, as seen developing on older leaf, (C) detail of chlorotic spots on leaf of TME204 3–4 weeks after graft initiation, (D) feathery chlorosis on veinal regions of leaf of TME204, (E) severe CBSD symptoms on leaf of cultivar TME204 approximately six weeks after graft initiation, (F) stem of cultivar 60444 showing severe symptoms of CBSD, (G) brown and grey cork lesions forming on stem tissue of CBSV infected cultivar TME204, (H) CBSD symptoms visible as brown colored streaks on the stem of cultivar Ebwanateraka, (I) brown lesions visible on outer surface of storage roots harvested from plants of cultivar 60444 approximately 2.5 months after chip bud graft inoculation and (J) typical CBSD systems seen within the storage parenchyma of tuberous roots 2.5 months after bud graft inoculation (left), symptom-free storage root from non-inoculated plant (right).