Efficient transmission of Cassava brown streak disease viral pathogens by chip bud grafting

BMC Research Notes

Table 2 Transmission of Cassava brown streak virus symptoms via bud grafts

	CBSV-free scion buds*	CBSV infected scion buds⁺
Cultivar	Successful grafts/number performed (%)	Successful grafts/number performed (%)	CBSV transmission/total grafts (%)
Ebwanateraka
1.	5/5	7/10	8/10
2.	5/5	9/10	10/10
60444
1.	5/5	8/10	9/10
2.	5/5	7/10	7/10
TME204
1.	5/5	9/10	10/10
2.	5/5	7/10	8/10
Total	30/30 (100)	47/60 (78.3)	52/60 (86.7)

*axillary buds were excised and grafted back onto the mother plant. Grafting success was assessed one week later and scored positive if the chip buds retained their green color and had attached to the rootstock with callus tissue formed along edges of the graft union.
⁺axillary buds were excised from cultivar Ebwanateraka plants infected with the Naliendele isolate of CBSV and grafted onto healthy plants of three different cassava cultivars. Transmission of CBSV was visually assessed for development of CBSD symptoms on leaves and stem of the rootstock test plant. Data is shown for plants six weeks after grafting in experiments performed twice (1 & 2).

ISSN: 1756-0500