From: Three multiplex snapshot assays for SNP genotyping in candidate innate immune genes
Gene | SNP (rs) | Forward (5’→3’) | Reverse (5’→3’) | Amplicon size (bp) | PCR mix (μM) |
---|---|---|---|---|---|
Panel 1 | |||||
IL1-α | 1800587 | GCAATAGACCTTATGACACCTAAC | GGTAGAGAAGAGAACAGTGGTATT | 731 | 3.0 |
IL1-β | 16944 | AGTATATGTGGGACAAAGTGGAAG | CTCATCTGGCATTGATCTGGTT | 389 | 1.4 |
IL6 | 1800795 | AGCCTGTTAATCTGGTCACTGA | CTTGTGGAGAAGGAGTTCATAGC | 498 | 3.2 |
IL10 | 1800872 | TTCTATGTGCTGGAGATGGTGTA | GGGTGGGCTAAATATCCTCAAAG | 385 | 1.4 |
IL12 | 11575934 | GGTTAAGTGACTGGTGCCAAG | TCCTGTACTCAGAGTGATCTTACC | 210 | 3.0 |
Panel 2 | |||||
TLR2 | 5743708 | TGCTGCCATTCTCATTCTTCCTG | TCCTCAAATGACGGTACATCCA | 282 | 1.4 |
IL10 | 1800896 | ATCTGAAGAAGTCCTGATGTCACT | ACCATCTCCAGCACATAGAATGA | 367 | 1.4 |
TLR2 | 4696480 | CATAGTTGTCACAGTCCCTTGG | TGGCATGATCTCGGCTCAC | 346 | 4.0 |
IL6 | 1800797 | GGTGAAGAGACTCAGTGGCAAT | AGTGACCAGATTAACAGGCTAGAAT | 444 | 2.0 |
TLR4 | 4986791 | CCGATTAGCATACTTAGACTACTACCT | AGACTGGACAAGCCATTGAAGAT | 555 | 3.2 |
Panel 3 | |||||
IL12 | 401502 | ACCTCTGTATGACATTGAGTAAGC | GAACCAACGGGACCACCAT | 285 | 1.0 |
TLR2 | 121917864 | CTGTGCTCTGTTCCTGCTGAT | GAAATGGGAGAAGTCCAGTTCATAC | 382 | 1.0 |
TLR9 | 187084 | ATTCATTCATTCAGCCTTCACTCAG | CCATCCAGCCTTCTTACAAACCT | 324 | 1.0 |
CD14 | 2569190 | GGCTTCCAGGCTTCACACT | AGGACACTGCCAGGAGACA | 236 | 1.0 |
TLR4 | 4986790 | TCCCTGAACCCTATGAACTTTATCC | AGGCTTGGTAGATCAACTTCTGAA | 494 | 1.0 |
CD14 | 2569191 | AAGGAAGGGATGGGACTATGTT | CAGGAATCTGAGGCAAGAGAAT | 347 | 1.0 |