Figure 5

Probe response to targets at two different hybridization temperatures of 55°C (represented in red) and 60°C (represented in Blue). Targets with complementary stretch of 9 to 21 nt were hybridized with surface attached probe at hybridization temperatures of 55°C and 60°C for 18 hrs followed by standard washing and drying procedure. Average background is indicated by dotted line. Probe response to perfect match at 55°C and 60°C are not significantly different (Data not shown). Error bars represent standard deviation across the replicate arrays.