Table 1 Vectors used for restriction enzyme mediated selectable marker gene disruption
Original Vector (and source) | Phenotype of original selectable marker | Cloning restriction site | Phenotype of cloned selectable marker | Construct name |
|---|---|---|---|---|
pUC19 (New England Biolabs) | ampR | Sca I | kanR | pUC19:kanR |
pUC19 (New England Biolabs) | ampR | Sca I | tetR | pUC19:tetA |
pZErO-2 (Invitrogen) | kanR | Nco I | tetR | pZErO-2:tetA |
pBlueScript SK + (Stratagene) | ampR | Sca I | kanR | pBlueScript SK+: kanR |
pET23a (Novagen) | ampR | Pst I | tetR | pET23a:tetA |