Figure 5From: Identification and enzymatic characterization of acid phosphatase from Burkholderia gladioliEffect of substrate concentration on phosphohydrolytic activity of membrane-bound acid phosphatase from Burkholderia gladioli . The PNPPase activity was assayed discontinuously at 37°C. Standard assessment conditions were 50 mM acetate buffer, pH 6.0, and containing increasing concentrations of substrate from 0.1 μM to 10 mM.Back to article page