Figure 3

Recovery of infectious chlamydiae after monocytes’ infection in presence of Apocynin or L-NAME. CT or CP infection was carried on in presence of Apocynin 300 μM or L-NAME 300 μM. As control, monocytes infection was carried on with no NADPH-oxydase or NOS inhibitors. At 24 hours post-infection, monocytes were disrupted and EBs infectivity was titrated in LLC-MK2 cells. At 3 days after the initial inoculum, chlamydial inclusions were enumerated. Since at 24 hours post-infection the viability of uninfected monocytes in presence of Apocynin or L-NAME was less than 30%, and similar low viability values were observed in CT or CP infected cells, with no significant differences, here recultivation results are presented as IFUs/100 cells in order to normalize the data obtained. A representative from three independent experiments is shown (mean ± standard error of the mean-SEM).