Figure 4

EGF induces ERK phosphorylation in human myometrial cells. Cells were stimulated with 25 ng/ml EGF for different time points (2-, 5-, 10-, and 20-min) in the absence or presence of 5 μM BIS1. (A) ERK phosphorylation was studied by means of immunoblotting using a phospho-ERK antibody. Blots were stripped and re-probed for ERK. (B) Fold change in ERK phosphorylation normalised to total ERK expression and non-treated values. Data points represent mean ± SEM, n = 3. Statistical significance was determined using two way ANOVA and Bonferroni post hoc test. * P < 0.05, **P < 0.01.