Figure 6

Mechanisms of PMA induced COX-2 expression in myometrial cells. Cells were stimulated for 6 hours with the PKC activator, PMA (2 μM) alone or in the presence of different inhibitors (TPCA-1 (2 μM), PD-184352 (2 μM) and BIS1 (5 μM)). (A) After lysis the cells were subjected to immunoblotting using a COX-2 antibody. Blots were stripped and re-probed for GAPDH. (B) COX-2 expression normalised to GAPDH expression and given as a percentage of the PMA treated value. Data points represent mean ± SEM, n = 5. Statistical significance was determined using one way ANOVA and Dunnett’s post hoc test. ***P < 0.001.