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Figure 2 | BMC Research Notes

Figure 2

From: Requirement of dual stimulation by homologous recombinant IL-2 and recombinant IL-12 for the in vitro production of interferon gamma by canine peripheral blood mononuclear cells

Figure 2

Evaluation of CTLL-2 proliferation following stimulation with recombinant canine IL-2 produced by E. coli or COS-7 cells. CTLL-2 cells were cultured for 48 h with: (A) different concentrations of affinity purified rcaIL-2-F43L produced in E. coli or (B) different concentrations of supernatant of COS-7 cells transfected with pcDNA3.1 without insert (circles) or pcDNA3.1-caIL-2 (squares). The cells were pulsed with 3H-thymidine for 24 h. The data represent the median of CPM of triplicates and the bars the 25 and 75 percentiles.

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BMC Research Notes

ISSN: 1756-0500

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