Table 1 Assembly statistics of Velvet applied on Staphylococcus aureus (MRSA) strain E-MRSA15-CC22-SCC mec IV showing an increase in contig size and N50 when using higher k-mer sizes, but revealing a mis-assembly starting from k-mer size 97 using whole genome mapping

Velvet

81

162295

40

340060

1 (10)

122303

83

170447

38

351373

1 (9)

122303

85

170449

37

351321

0 (10)

87

173763

33

351326

0 (10)

89

173765

33

351394

0 (10)

91

173767

33

351330

0 (10)

93

173769

35

340092

0 (10)

97

175770

33

365247

1 (9)

130273

99

175776

33

365260

1 (10)

130273

101

187438

32

365623

1 (9)

130273

103

187448

32

365625

1 (9)

130273

105

187458

32

365638

1 (9)

130273

107

187465

33

365647

1 (9)

130273

109

212189

32

365656

1 (9)

130273

111

212287

33

349286

2 (8)

93632 & 153207

113

212292

34

349288

1 (10)

93634

115

212294

34

349290

1 (10)

118928

117

174074

35

349419

1 (11)

93634

119

174076

35

349423

1 (11)

93638

121

170642

37

349435

0 (11)

123

170654

38

340456

0 (10)