Example of behaviour of the algorithms developed for implementation of slope method. Behaviour of the algorithms developed for implementation of SM. Estimated regression lines are shown superimposed on a simulated noisy Cl curve (the same reported in Figure 2). From the top: a) semiautomatic algorithm, black stars represent start and end points selected from the operator; b) automatic algorithm; c) automatic algorithm after smoothing. In b and c the black stars represent the point automatically recognised from the algorithm as sample at maximum slope. In this case, computed BFa were respectively: 68.55, 78.30 and 123.81 ml/min/100 ml.