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Table 2 Expression stability and ranking of ten reference genes in each strains derived-embryos analyzed using NormFinder software

From: Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real-time quantitative RT-PCR (RT-qPCR)

Strains

Ranking

In vitro

In vivo

 

KSOM

CZB

M16

Total

 

B6D2F-1

1

h2afz

h2afz

wrnip

h2afz

h2afz

BC57BL/6

h2afz

h2afz

gapdh

h2afz

h2afz

ICR

gapdh

ablim

sptbn

h2afz

sptbn

B6D2F-1

2

actb

18 s

18 s

18 s

gapdh

BC57BL/6

sdha

gapdh

h2afz

gapdh

gapdh

ICR

h2afz

actb

h2afz

ablim

h2afz

B6D2F-1

3

tgfb1

sptbn

h2afz

sptbn

tgfb1

BC57BL/6

tgfb1

actb

sptbn

sdha

18 s

ICR

ablim

h2afz

ablim

sdha

ywhaz

B6D2F-1

4

ablim

gapdh

gapdh

tgfb1

wrnip

BC57BL/6

gapdh

tgfb1

actb

sptbn

wrnip

ICR

18 s

sdha

sdha

tgfb1

ablim

B6D2F-1

5

sptbn

sdha

ablim

actb

sptbn

BC57BL/6

actb

sptbn

sdha

tgfb1

tgfb1

ICR

sdha

tgfb1

tgfb1

gapdh

actb

B6D2F-1

6

18 s

wrnip

tgfb1

wrnip

18 s

BC57BL/6

sptbn

18 s

ywhaz

actb

sptbn

ICR

sptbn

gapdh

18 s

sptbn

18 s

B6D2F-1

7

wrnip

tgfb1

actb

gapdh

ablim

BC57BL/6

ablim

sdha

wrnip

ywhaz

actb

ICR

tgfb1

ywhaz

actb

18 s

tgfb1

B6D2F-1

8

ywhaz

ywhaz

sptbn

ablim

ywhaz

BC57BL/6

ywhaz

ywhaz

18 s

18 s

ablim

ICR

ywhaz

wrnip

ywhaz

ywhaz

wrnip

B6D2F-1

9

gapdh

actb

ywhaz

sdha

sdha

BC57BL/6

wrnip

wrnip

tgfb1

wrnip

ywhaz

ICR

wrnip

sptbn

gapdh

actb

gapdh

B6D2F-1

10

sdha

ablim

sdha

ywhaz

actb

BC57BL/6

18 s

ablim

ablim

ablim

sdha

ICR

actb

18 s

wrnip

wrnip

sdha