Haplotype defined by the MLH1-93G/A polymorphism is associated with MLH1 promoter hypermethylation in sporadic colorectal cancers

Table 4 Association between haplotype of seven SNPs including MLH1-93G/A SNP and the methylation status of the MLH1 promoter region

<Total cases > Haplotype¹	Haplotype frequencies²			χ²-value	p-value³
<Total cases > Haplotype¹	Total	M-negative	M-positive	χ²-value	p-value³
C-A-T-A-A-A-C	0.3141	0.3031	0.3431	0.6274	0.4283
A-A-T-A-G-A-C	0.3116	0.3366	0.2499	2.9665	0.085
A-A-T-A-A-A-C	0.198	0.1667	0.2755	6.3116	0.012
C-T-C-C-G-G-A	0.149	0.1644	0.1102	1.9578	0.1618
C-A-T-A-G-A-C	0.0273	0.0291	0.0213	0.1968	0.6573
<Female cases > Haplotype¹⁾	Haplotype frequencies²			χ²-value	p-value³
<Female cases > Haplotype¹⁾	Total	M-negative	M-positive	χ²-value	p-value³
C-A-T-A-A-A-C	0.2983	0.3046	0.2955	0.0154	0.9014
A-A-T-A-G-A-C	0.2801	0.3346	0.2017	3.3986	0.0653
A-A-T-A-A-A-C	0.2077	0.1154	0.3452	12.6416	0.00038
C-T-C-C-G-G-A	0.1829	0.21	0.1406	1.2578	0.2621
C-A-T-A-G-A-C	0.0309	0.0354	0.017	0.4818	0.4876
<Male cases > Haplotype¹	Haplotype frequencies²			χ²-value	p-value³
<Male cases > Haplotype¹	Total	M-negative	M-positive	χ²-value	p-value³
C-A-T-A-A-A-C	0.3323	0.3381	0.3105	0.1457	0.7027
A-A-T-A-G-A-C	0.3243	0.3028	0.4031	1.9442	0.1632
A-A-T-A-A-A-C	0.1916	0.1922	0.1895	2.00E-03	0.9643
C-T-C-C-G-G-A	0.127	0.1414	0.0741	1.7339	0.1879
C-A-T-A-G-A-C	0.0249	0.0255	0.0229	0.0117	0.9138

¹Loci of SNPs examined were as follows: rs2276807 - rs4678922 - rs6789043 -rs1046512- rs1800734 (bold letter) - rs3774343 - rs4647215.
²Total cases, methylation-negative (M-negative) cases and methylation-positive (M-positive) cases of haplotype frequencies.
³P values of <0.05 were statistically significant.

ISSN: 1756-0500